An interesting video on this scientists experience during a stroke that allowed her to better understand what appears to be affects of the left and right brains.
Wednesday, October 26, 2011
Tuesday, October 11, 2011
Extensive chromatin fragmentation improves enrichment of protein binding sites in chromatin immunoprecipitation experiments
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2577354/
As you guys know, yesterday I performed quite a few sonications and I started looking (again, as I do often) at papers that indicate the optimal size for sonicated DNA.
I found this paper. And it makes sense. It basically points out that with more sonications (~200bp) you increase the fold enrichment of your target by reducing the background or non-specific binding. But, you'll ip less (and probably purify less because the %efficiency we get back from the kits goes down with smaller fragments).
Happy ChIP'in!
As you guys know, yesterday I performed quite a few sonications and I started looking (again, as I do often) at papers that indicate the optimal size for sonicated DNA.
I found this paper. And it makes sense. It basically points out that with more sonications (~200bp) you increase the fold enrichment of your target by reducing the background or non-specific binding. But, you'll ip less (and probably purify less because the %efficiency we get back from the kits goes down with smaller fragments).
Happy ChIP'in!
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